Additional file 2: Figure S1. of MicroRNA-195 acts as an anti-proliferative miRNA in human melanoma cells by targeting Prohibitin 1

MicroRNA-195 modulates PHB1 expression in melanoma cells. SK-MEL-5 melanoma cells were transfected with either miR-control/mir-195. miR-195 mimics transfection resulting in a reduction of PHB1 (P ≤ 0.01) (a) mRNA and (b) protein levels compared to miR-control. For RT-qPCR experiments, ACT-B mRNA was used as an endogenous control and the data were analyzed using the 2 (−∆∆Ct) method; for immunoblotting ACT-B was also used as loading control. Protein quantification (fold-change based on the control) is indicated above the blots. In (c) and (d), miR-195 levels 48 and 72 h after transfection, respectively. RNU48 was used as an endogenous control and the data were analyzed using the 2 (−∆∆Ct) method. **P ≤ 0.01. (PNG 74 kb)