Additional file 2: Figure S1. of Intravitreal administration of multipotent mesenchymal stromal cells triggers a cytoprotective microenvironment in the retina of diabetic mice

Donor MSCs differentiate into neural- or perivascular-like cells at very low frequency. One, 4, 8 and 12 weeks after MSC administration immunofluorescences were performed in eye sections to evaluate differentiation of MSCs into RGCs by colocalization of GFP with the ganglion cell marker β3-tubulin (A) or differentiation into astrocytes by colocalization of GFP with the astrocyte marker GFAP (B). To evaluate differentiation of MSCs into perivascular cells, immunofluorescences were performed to colocalize GFP with the pericyte marker NG2 in whole mount retinal preparations. Lectin was also added to detect blood vessels. Arrows indicate cells co-expressing both markers. Qualitative data are representative of six eyes per analyzed time. (PNG 2819 kb)