Additional file 2: Figure S1. of Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

Quantification of miRNAs by RT-qPCR. Validation of (A) Nb-miRNA167 and (B) Nb-miR482 expression by RT-qPCR. Northern blot validation of conserved miRNAs (Fig. 4.) compared to RT-qPCR quantification. In both experiments the reference gene was U6. (PDF 134 kb)