13058_2016_672_MOESM2_ESM.pdf (213.75 kB)
Additional file 2: Figure S1. of Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers
journal contribution
posted on 2016-01-25, 05:00 authored by Alison Ward, Paul Mellor, Shari Smith, Stephanie Kendall, Natasha Just, Frederick Vizeacoumar, Sabuj Sarker, Zoe Phillips, Riaz Alvi, Anurag Saxena, Franco Vizeacoumar, Svein Carlsen, Deborah AndersonCREB3L1 protein expression in a panel of breast cancer cell lines. Cell lysates (50 μg protein/lane) from the indicated breast cancer cell lines were probed for CREB3L1 and β-actin (loading control). The full-length precursor form (approximately 84 kDa) and processed mature form (56–58 kDa) of CREB3L1 are indicated (arrows). A control lysate from HCC1806 cells transfected with HA-tagged CREB3L1 was also included (HA-CREB3L1). It has both the precursor (P) and cleaved, mature (M) forms of CREB3L1, and as a result of the triple HA-tag, they are slightly larger in size (approximately 6 kDa) than the endogenous CREB3L1 proteins. *Background band. (PDF 213 kb)
