Additional file 2: Figure S1. of Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers

CREB3L1 protein expression in a panel of breast cancer cell lines. Cell lysates (50 μg protein/lane) from the indicated breast cancer cell lines were probed for CREB3L1 and β-actin (loading control). The full-length precursor form (approximately 84 kDa) and processed mature form (56–58 kDa) of CREB3L1 are indicated (arrows). A control lysate from HCC1806 cells transfected with HA-tagged CREB3L1 was also included (HA-CREB3L1). It has both the precursor (P) and cleaved, mature (M) forms of CREB3L1, and as a result of the triple HA-tag, they are slightly larger in size (approximately 6 kDa) than the endogenous CREB3L1 proteins. *Background band. (PDF 213 kb)