Additional file 2: Figure S1. of Characterization of osteoarthritic human knees indicates potential sex differences

Phenotypic characteristics of female and male chondrocytes isolated from osteoarthritic knees. First-passage chondrocytes were treated with 10−7 M 24R,25(OH)2D3 (A-D) or 10-8 M DHT (E–H). Alkaline phosphatase-specific activity (A, D) was measured in whole cell lysates. mRNAs for chondrocyte genes aggrecan (B, F), type-II collagen (C, G), and cartilage oligomeric matrix protein (D, H) were measured using real-time qPCR. Data show treatment compared with vehicle control ratios of the responses of 6 male and 6 female patients. The dashed line represents the vehicle control (dashed line = 1). * p < 0.05 vs. control; † p < 0.05 vs. male. (TIF 1363 kb)