Additional file 2: Figure S1. of A high-throughput pipeline for detecting locus-specific polymorphism in hexaploid wheat (Triticum aestivum L.)

Validation of marker location of AEGTA18760 using a RIL (recombination inbred lines) population. Orthologous sequences of AEGTA18760 were amplified from the two parents of the RIL population, C (‘CSCR6’) and L (‘Lang’), and sequenced. The single nucleotide polymorphism (in red and green) and restriction enzyme sites (underlined) were identified between C and L for AEGTA18760 with restriction enzyme NlaIII (A). The amplified products of the two parents and 13 of the RIL lines were digested and separated on agarose gels. The map position of the new marker on chromosome 3B (B) was calculated based on the linkage map published by Ma et al. [22].