Additional file 2: Figure S1 A, B and C. of Phylogenetic assessment reveals continuous evolution and circulation of pigeon-derived virulent avian avulaviruses 1 in Eastern Europe, Asia, and Africa

Mismatches between the tested viruses and: A) previous fusion probe designed by Wise et al. [24]; B) pigeon-specific fusion probe designed by Kim et al. [66]; and C) the optimized pigeon specific probe in this study, respectively. Sequences are in order of 5′ to 3′. Figure S2. A and B Mismatches between the tested viruses and: A) previous fusion forward primer designed by Wise et al. [24]; and B) the optimized fusion forward primer in this study, respectively. Sequences are in order of 5′ to 3′. Figure S3. A and B Mismatches between the tested viruses and: A) previous fusion reverse primers designed by Wise et al. [24]; B) and the new fusion reverse in this study, respectively. Sequences are in order of 5′ to 3′. Figure S4. Phylogenetic analysis based on the complete genome concatenated coding sequence of viruses representing NDV class II. Only bootstrap values greater or equal to 60% are visualized. There were a total of 13,697 positions in the final dataset. The strains sequenced in this study are highlighted in bold font and have a circle symbol in front the taxa name. Provisional designation of genotypes is indicated on the right. (DOCX 1990 kb)