40168_2020_790_MOESM26_ESM.pdf (175.03 kB)
Additional file 26 of A pipeline for targeted metagenomics of environmental bacteria
journal contribution
posted on 2020-03-24, 12:59 authored by Anissa Grieb, Robert M. Bowers, Monike Oggerin, Danielle Goudeau, Janey Lee, Rex R. Malmstrom, Tanja Woyke, Bernhard M. FuchsAdditional file 26: Figure S15. Exemplary scatter plots from flow cytometry showing the gating principle of seawater samples hybridized with HCR-FISH probe. (A) DAPI positive cells were selected in the forward scatter vs. blue fluorescence (DAPI signal) plot and (B) HCR-FISH positive cells were selected in the blue vs. green fluorescence (HCR-FISH signal) plot. Of (B) only those events that were also appearing in the gate in (A) were sorted.