12915_2017_361_MOESM1_ESM.eps (7.53 MB)
Additional file 1: of Giardia intestinalis mitosomes undergo synchronized fission but not fusion and are constitutively associated with the endoplasmic reticulum
figure
posted on 2017-04-03, 05:00 authored by Luboš Voleman, Vladimíra Najdrová, Ásgeir Ástvaldsson, Pavla Tůmová, Elin Einarsson, Zdeněk Švindrych, Guy Hagen, Jan Tachezy, Staffan Svärd, Pavel DoležalDividing mitosomes in mitotic G. intestinalis. G. intestinalis culture expressing IscU-Halo was enriched for mitotic trophozoites by albendazole treatment (100 ng/mL) for 6 h at 37 °C. The cells were washed twice in warm medium and stained by Halo-TMR ligand and observed under a microscope. The images are representative of the sequence submitted as movie files (Additional files 2, 3, and 4). (EPS 7706 kb)