Additional file 1: of Xenografting of human umbilical mesenchymal stem cells from Wharton’s jelly ameliorates mouse spinocerebellar ataxia type 1

Figure S1. Chromosomal karyotyping and surface markers of HUMSCs in vitro. To analyze the copy number of 23 chromosomes of HUMSCs in vitro (passage 10th), the CytoScan 750 K Array (Affymetrix) was used to screen the chromosomal karyotype. The X-axis represents the chromosome number (No. 1–22, XX), the right Y-axis represents the copy number of chromosome (0, 1, 2, or 3), and the blue line is the copy number of chromosomes performed by the company of Genetics Generation Advancement. The result indicated that the chromosomes No. 1–22 and sex chromosome (X) are all two sets. (B) Flow cytometry analyses of surface markers of HUMSCs in vitro. HUMSCs were cultured for 10 passages and then labeled with CD44, CD105 and HLA-DR antibodies. White areas represent negative controls and red areas represent the specific binding for indicated antigens. The results revealed that HUMSCs transplanted into mice were positive for CD44 and CD105 but negative for HLA-DR. (PDF 142 kb)