Additional file 1 of The novel long noncoding RNA CRART16 confers cetuximab resistance in colorectal cancer cells by enhancing ERBB3 expression via miR-371a-5p
2020-03-05T05:01:43Z (GMT) by
Additional file 1: Figure S1. Phenotypic characteristics of Caco-2 and Caco-2 CR cells. a Flow cytometry was performed in Caco-2 and Caco-2 CR cells with cetuximab treatment (100 μg/ml and 200 μg/ml) for 48 h. APC Annexin V−/7-AAD− denotes live cells; APC Annexin V+/7-AAD− denotes early apoptotic cells; APC Annexin V−/7-AAD+ denotes necrotic cells; and APC Annexin V+/7-AAD+ denotes late apoptotic cells. b The cell cycle was assessed by flow cytometry in Caco-2 and Caco-2 CR cells after 48 h of treatment with cetuximab (200 μg/ml). c The percentage of EGFR-, ERBB3-, and c-MET-positive cells and the MFI were determined by GALLIOUS flow cytometry in Caco-2 and Caco-2 CR cells. d Flow cytometry analysis showed the expression of stemness biomarkers of CRC cells, CD44 and CD133, in Caco-2 and Caco-2 CR cells.