Additional file 1: of The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status

Figure S1. Morphological evaluation of the maturational status in male salmon. Representative histology (hematoxylin/eosin combined with periodic acid according to Schiff, H/E-PAS; (A-C)) and proliferation activity (immune-detection of phosphorylated histone H3, pH 3; (D-F)) in testes tissue obtained from Atlantic salmon in different stages of maturation. Representative type A undifferentiated (black arrowheads), type A differentiating (black dashed lines), type B spermatogonia (white dashed lines), and “free” Sertoli cells (black arrows) are shown in B-C. In E-F, representative pH 3-positive cells are indicated by red arrowheads (type A undifferentiated spermatogonia), red dashed lines (type B spermatogonia) and red arrows (“free” Sertoli cells), respectively. Scale bar represents 25 (A) or 30 (B-F) μm. (TIF 6792 kb)