Additional file 1: of The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells

Figure S1. Analysis of Sox2-null ES cells rescued by wild-type Sox factors. A Morphology of primary transfectants with Sox6 and Sox7. Transfection of Sox6 expression vectors caused differentiation of large flat trophectoderm-like cells, whereas with Sox7 spindle cells resembling parietal endoderm were occasionally observed. B Immunostaining of Sox2-null ES cells rescued by Sox1 with anti-Sox1. Ectopic expression of Sox1 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. C Immunostaining of Sox2-null ES cells rescued by Sox3 with anti-Sox3. Ectopic expression of Sox3 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. D Immunostaining of Sox2-null ES cells rescued by Sox15 with anti-Sox15. Ectopic expression of Sox15 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. (PDF 2664 kb)