Additional file 1: of The effects of mepiquat chloride on the lateral root initiation of cotton seedlings are associated with auxin and auxin-conjugate homeostasis

Figure S1. Changes in root, shoot biomass and shoot/root ratio at 12 (A) and 20 (B) d after MC treatment. Figure S2. The effect of MC on IAA distribution in LRI priming region of cotton roots at 3 d after MC treatment. Figure S3. The expression of IAA amidohydrolase genes in the cotton seedlings. Figure S4. Amino acid sequence alignment of IAHs in cotton (Gh) and Arabidopsis (At). Figure S5. Amino acid sequence alignment of ILR1 in Gossypium hirsutum (CGP-BGI Assembly: CotAD_; NAU-NBI Assembly: Gh_A/Gh_D; Ncbi: XP_.) and Gossypium raimondii (JGI assembly: Gorai.). Figure S6. Amino acid sequence alignment of IAR3a in Gossypium hirsutum, Gossypium arboretum (BGI Assembly: Cotton_A) and Gossypium raimondii (BGI-CGP v1.0 Assembly: Cotton_D). Figure S7. Amino acid sequence alignment of IAR3b in Gossypium hirsutum, Gossypium arboretum and Gossypium raimondii. Figure S8. Purified fusion protein GST-IAR3a, GST- IAR3b and His-ILR1 used in activity assays (A) and GhIAR3a was separated as the antigen (B). Figure S9. Cross-Reactivity of mAb 3D11 and rabbit antisera against to GhIAR3a with GST (A), GhILR1 (B) and GhIAR3b (C). (DOCX 2404 kb)