Additional file 1: of Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

Figure S1. Breeding blocks and sampling. Figure S2. Representative examples of the four categories of gonad categorization. Figure S3. EE2 concentrations in EE2-treated 200L tanks over time. Figure S4. Impact of DNA contamination on transcriptome analysis. Figure S5. Differential gene expression of control and EE2-treated males or females at different developmental stages. Figure S6. Frequencies of undifferentiated juveniles in control and EE2-treated groups. Table S1. Top 25 gene ontology terms enriching the genes of EE2-treated males at embryo stage. Table S2. Top 25 gene ontology terms enriching the genes of EE2-treated males at hatching. Table S3. Top 25 gene ontology terms enriching the genes of EE2-treated females at hatching. Table S4. Top 25 gene ontology terms enriching the genes of EE2-treated females at first feeding. Table S5. Results for classical sex-related genes. Table S6. Likelihood ratio test on rate of undifferentiated gonads over time and treatment. Table S7. RNA quality and RNA-sequencing. (DOCX 4195 kb)