Additional file 1: of Selective killing of circulating tumor cells prevents metastasis and extends survival

Changes in colony formation according to the time elapsed since the intravenous injection of NCI-H460 cancer cells. a Change in colony formation according to the time elapsed since cancer cell injection. NCI-H460 cells (1 × 105) were intravenously injected into mice and whole blood was collected by cardiac puncture. After lysis of the red blood cells, 200 μL was spread onto a 35-mm dish and incubated for 7 days. The clone numbers were counted using crystal violet staining. The minutes represent the time passed between the cell injection and the blood collection. b The change in cancer cell colony number expressed as a graph. ns, not significant; **, P < 0.01. (PDF 45 kb)