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Additional file 1: of Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins

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posted on 05.04.2019, 05:00 by Robert Mitchell, Ben Mellows, Jonathan Sheard, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, James Tomkins, Bernd Denecke, Luca Musante, Barbara Joch, Florence Debacq-Chainiaux, Harry Holthofer, Steve Ray, Tobias Huber, Joern Dengjel, Paolo Coppi, Darius Widera, Ketan Patel
Figure S1. ADSCs retain their multipotency following secretome generation. (A–B) FACS analysis before and after generation of the secretome revealed that there was no change in expression pattern of ADSCs after 24 h in PBS. (C) Surviving cells readily differentiated into Alizarin Red-positive osteogenic cells, (D) Oil Red O-positive adipocytes, and (E) Alcian blue-positive chondrogenic cells. Scale bar: 100 μm. (JPG 339 kb)

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