Additional file 1: of Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins

Figure S1. ADSCs retain their multipotency following secretome generation. (A–B) FACS analysis before and after generation of the secretome revealed that there was no change in expression pattern of ADSCs after 24 h in PBS. (C) Surviving cells readily differentiated into Alizarin Red-positive osteogenic cells, (D) Oil Red O-positive adipocytes, and (E) Alcian blue-positive chondrogenic cells. Scale bar: 100 μm. (JPG 339 kb)