Additional file 1: of Retinal ischemia induces α-SMA-mediated capillary pericyte contraction coincident with perivascular glycogen depletion

Figure S1. Constricted microvascular segments on the ischemic/recanalized whole-mount retinae. Retinae stained with vascular markers claudin-5 (A, C) or lectin (B) illustrate the microvascular constrictions (arrows). These images are the green channel of the merged images in Fig. 2c-e in the main text. (D) Scrambled siRNA injected 24 h before ischemia as a control for α-SMA siRNA did not modify α-SMA expression (red) nor prevent ischemia-induced constrictions (arrows). Vessels were labeled with lectin (green). Scale bar in A-B = 10 μm; scale bar in C = 5 μm; ; in D = 25 μm. (TIF 4329 kb)