Additional file 1: of Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

DNA purification reagents vary in their ability to recover low amounts of DNA. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 5, 10, 50 ng range of DNA in ChIP elution buffer was purified following the manufacturer’s instructions as described in Fig. 1a. The data were generated from triplicate DNA samples derived from three independent preparations. b Recovery rates of nanogram-range DNA by different DNA purification reagents. DNAs were adjusted to final 1 ng, 5 ng, 10 ng and 50 ng in 100 μL ChIP elution buffer and were purified by purification reagents following the manufacturer’s instructions. Percent recovery was calculated from DNA amounts before and after purification. The data were generated from triplicate DNA samples derived from three independent preparations. (PDF 105 kb)