12868_2020_554_MOESM1_ESM.pptx (5.47 MB)
Additional file 1 of Proximity ligation assay reveals both pre- and postsynaptic localization of the APP-processing enzymes ADAM10 and BACE1 in rat and human adult brain
presentation
posted on 2020-02-05, 04:49 authored by Jolanta Lundgren, Lina Vandermeulen, Anna Sandebring-Matton, Saheeb Ahmed, Bengt Winblad, Monica Di Luca, Lars Tjernberg, Elena Marcello, Susanne FrykmanAdditional file 1. Proximity ligation assay showing co-localization of ADAM10 and BACE1 with their substrate APP in adult rat brain. Adult rat brain hippocampal sections were subjected to PLA and each signal (brown dot) generated denotes two proteins within 40 nm distance from each other. (a) APP and ADAM10, (b) APP and BACE1. (c) Negative control with no primary antibodies. Brain tissue and cell nuclei were visualized by a nuclear stain solution containing Mayer´s haematoxylin. Each experiment was performed three times and representative images are shown. Scale bar 20 µm.