Additional file 1: of Porcine circovirus type 2 ORF3 protein induces apoptosis in melanoma cells

Figure S1. Transfection efficiency. To find out the most effective way for overexpression of recombinant proteins DNA was introduced into all cell lines by transfection with Lipofectamine (LTX) or polyethyleneimine (PEI). Consequently, the DNA: LTX and DNA: PEI complex penetrate cell membranes and recombinant proteins are produced. After 24–48 h of transfection, cells were harvested, and transfection efficiency of every experiment was determined using parallel wells that were transfected only with the plasmid encoding green flourescent protein (GFP). Since LTX was more effective transfection agent compared to PEI in all used cell lines, it was used in subsequent experiments. (PDF 417 kb)