Additional file 1: of Physiological and transcriptional analyses of developmental stages along sugarcane leaf

It is comprised by 13 Additional figures and methodologies that complement the data presented in the manuscript. Figure S1. Distribution of leaf length. Figure S2. Phenotype of 60 days-old plants. Figure S3. Planning for sample collection. Additional Methodology of leaf gas exchange and photochemistry evaluation on the B and M segments: on this supplemental topic we described the methodology used to produce Additional Fig. 4 and 5. Figure S4. Light response curves for different leaf segments. Figure S5. Responses of leaf CO2 assimilation to increasing intercellular CO2 concentration (Ci) for different leaf segments. Figure S6. Stomata counting in 2 cm segments of sugarcane leaf. Figure S7. Venn Diagram representing groups of orthologous genes shared between the five species (Sorghum bicolor, Setaria italica, Saccharum sp. SP80-3280, Oryza sativa and Zea mays). Figure S8. Protein content of sugarcane leaf segments. Figure S9. Expression patterns exhibited by the 14 genes identified in all contrasts (Figure 5 on the manuscript). Figure S10. Water content. Figure S11. Phylogenetic tree of the Trehalose 6-Phosphate Synthase family. Figure S12. Expression pattern of cell wall related genes. Figure S13. Expression pattern of genes peaking at segment M. Additional References. References of the Methodology of Gas exchange and fluorescence evaluation. (DOCX 6742 kb)