Additional file 1: of Phylogeny, Divergent Evolution, and Speciation of Sulfur-Oxidizing Acidithiobacillus Populations

Figure S1. Geographic distributions and genome attributes of Acidithiobacillus strains used for the construction of genome-based phylogeny and the calculation of average nucleotide identity. Figure S2. Substitution pattern of 16S rRNA genes used for phylogenetic tree. The number of transitions (cross) and transversions (triangle) against the TN93 distance is shown in different colors. Each point in individual sites indicates a pairwise comparison between two of taxa. Figure S3. Calculations of ANIb (upper half) and ANIm (lower half) for Acidithiobacillus strains. The values of ANI above the threshold for species delineation (95%) are highlighted. Table S1. Statistics for the number of RNA (rRNA and tRNA) in Acidithiobacillus (A.) strains with available genomes. Table S3. Functional classifications of the common genes shared by Acidithiobacillus strains using an online platform KAAS. Table S4. Summary for gene families in the genomes of Acidithiobacillus strains, including A. thiooxidans (formerly A. albertensis) DSM 14366 (1), A. caldus strains ATCC 51756 (2), DX (3), MTH-04 (4), SM-1 (5), ZBY (6), ZJ (7), GGI-221 within A. ferrooxidans (formerly Acidithiobacillus sp.) GGI-221 (8), Acidithiobacillus sp. SH (9), A. ferrooxidans strains ATCC 23270 (10), ATCC 53993 (11), Hel18 (12), YQH-1 (13), Acidithiobacillus sp. (formerly A. ferrooxidans) BY0502 (14), A. ferrivorans strains CF27 (15), PRJEB5721 (16), SS3 (17), YL15 (18), A. thiooxidans strains A01 (19), A02 (20), BY-02 (21), CLST (22), DMC (23), DXS-W (24), GD1-3 (25), JYC-17 (25), Licanantay (27), and ZBY (28). (DOC 37024 kb)