Additional file 1: of Peripheral infusion of human umbilical cord mesenchymal stem cells rescues acute liver failure lethality in monkeys

Table S1. General information and treatments of monkeys. Table S2. hUC-MSCs quality inspection report: viral factors and pathogens. Figure S1. The surface markers and multiple differentiation potentialities of hUC-MSCs. (A) Flow cytometric analysis of the cell markers of mesenchymal stem cells. (B) Adipogenic differentiation and osteogenic differentiation of hUC-MSCs were determined by Oil red O staining and alizarin red staining. Scale bar = 50 μm. Figure S2. The rhesus monkeys completely tolerated the xenogeneic hUC-MSCs. (A) Schematic representations of the experimental designs. (B) Sum and sort counting of leukocytes. (C, D) Flow cytometric analysis and quantitation of the ratio of the CD4+/CD8+ cells. (E, F) Flow cytometric analysis and quantitation of the proportion of CD4+CD25+FoxP3+ regulatory T cells. (G) Serum levels of IgA, IgG, and IgM over time. Each bar represents the mean ± s.e.m., n ≥ 3. Figure S3. hUC-MSC infusion does not change peripheral leukocytes, ratio of CD4+/CD8+ T cell, regulatory T cells and DCs. (A) Flow cytometric analysis and quantitation of the ratio of CD4+/CD8+ T cells. (B) Flow cytometric analysis of CD4+/CD25+FoxP3+ regulatory T cells. (C) Flow cytometric analysis of CD1a+/CD80+/CD86+ dendritic cells. Each bar represents the mean ± s.e.m., n ≥ 5/group. Figure S4. hUC-MSC infusion does not disturb antibodies and complements. Student’s t test, the data are presented as the means±s.e.m., n ≥ 5. Figure S5. Heat map of altered genes from microarray analysis. ArrayExpress accession number: E-MTAB-4750, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4750/ . (DOCX 3772 kb)