Additional file 1: of Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis

Figure S1. Schematic of TBCP-1 model development and metastasis assays. (A) Parental TBCP-1 cells were derived from long-term culture of a spontaneously arising mammary tumour from a BALB/C mouse (SMT1). Clonal lines isolated by FACS were selected based on spontaneous metastatic abilities in vivo and phenotype analysis to generate the brain metastatic TBCP-1 line. The morphology of parental and clonal TBCP-1 cells in standard culture is shown in the bottom left panels. Scale bar = 50 μm. Expression of EGFR/HER1, HER2, HER3 and HER4 in TBCP-1 and SKBR3 cells determined by western blotting is shown in bottom middle panels. HER2 membrane expression in TBCP-1 cells was detected by standard flow cytometry (right panel). (B) Experimental metastasis assay. (C) Spontaneous metastasis assay. (TIF 16222 kb)