Additional file 1: of NFAT5 mediates hypertonic stress-induced atherosclerosis via activating NLRP3 inflammasome in endothelium

Supplementary Materials and Methods. Figure S1. Effect of high salt intake on weight and atherosclerotic lesions formation of ApoE-/- mice. Figure S2. HUVECs adapt well in hypertonic medium. Figure S3 High-salt elevates the expression of vWF in HUVECs. Figure S4. Overexpression of NFAT5 increases Caspase-1 activity in ECs by treated with Ad-null, 5 MOI, Ad-NFAT5, 2.5 MOI or 5 MOI. Figure S5. High Salt-elevated NFAT5 mediate ROS production in ECs via. Figure S6. NFAT5 mediates IL-1β expression. Table S1. The primers sequences for RT-qPCR in this study. Table S2. Effect of high-salt intake on serum level of ApoE-/- mice. (DOCX 1785 kb)