Additional file 1: of N-acetylcysteine decreases malignant characteristics of glioblastoma cells by inhibiting Notch2 signaling

Figure. S1 NAC decreases protein levels of Notch2, Notch3 signaling. A, B, C and D, The relative expression levels of Notch2 and Notch3 were analyzed by western blot. U87 and U251 cells were treated with NAC (2, 5, 10 or 20 mM) for 24 h (A and B) or with NAC (10 mM) for 3, 6, 12, 24 or 48 h (C and D). E, F, G and H, The relative expression levels of Hes1 and Hey1 were analyzed by western blot. U87 and U251 cells were treated with NAC (2, 5, 10 or 20 mM) for 24 h (E and F) or with NAC (10 mM) for 3, 6, 12, 24 or 48 h (G and H). β-actin was used as a loading control. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with control group. (TIF 5225 kb)