Additional file 1: of Modulation of astrocyte reactivity improves functional deficits in mouse models of Alzheimer’s disease

Figure S1. AAV infect astrocytes selectively. To validate astrocyte tropism of the AAVs used in our study, an AAV2/9 encoding GFP was injected in the hippocampus of WT mice. GFP+ cells co-express the astrocytic marker GFAP and S100β, but not NeuN, IBA1, Olig2 and MBP, which are markers of neurons, microglial cells, cells of the oligodendrocyte lineage and myelinating oligodendrocytes, respectively. Astrocyte tropism of these vectors was confirmed in AD mice as well (See colocalization of GFP with GFAP in Figs. 1b and S2). (TIF 14477 kb)