Additional file 1: of Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig

Processes of ramified microglia primarily pass over normal myelinated fibers in the sham thalamus. Movie representing 3D reconstructions of confocal z-stacks of thalamic sites from sham-injured animals, in which Dapi-labeled nuclei are in blue, MBP+ myelinated axons are in red, and Iba-1+ microglia are in white. Purple circles highlight contacts between microglial processes and normal myelinated fibers. Notice that while only a few microglial processes come in direct apposition with normal myelinated fibers, the majority of microglial processes that do contact myelinated fibers do not terminate at the axon; rather pass over the myelinated fiber. Scale bar: 10 Οm. (MP4 16442 kb)