Additional file 1: of Microglia affect α-synuclein cell-to-cell transfer in a mouse model of Parkinson’s disease

Figure S1. Reduction of microglia using the specific microglia marker TMEM119 and striatal levels of huα-syn, pS129 α-syn, LC3B, LAMP1 and p62 in control and PLX-treated mice. a Western blots of ipsilateral (I) and contralateral (C) striatal tissue from control and PLX-treated mice were probed for pS129 α-syn, huα-syn and autophagy makers LC3B-1, LAMP1 and p62 normalized to β-actin. Pon S for loading reference. b Quantification of panel a (control, circles, n = 3–6, PLX, squares, n = 4–8). Error bars represent S.E.M. Pon S; Ponceau S, Str; striatum. c Cortex and striatum images from control and PLX-treated mice indicating reduced TMEM 119-postivie microglia in PLX-treated mice, scale bar 50 μm. (TIF 138905 kb)