Additional file 1: of Methylglyoxal, a glycolysis metabolite, triggers metastasis through MEK/ERK/SMAD1 pathway activation in breast cancer

Supplementary methods: RNA sequencing, shRNA transfection, siRNA transfection, Plasmids transfection, Immunofluorescence, RNA isolation and quantitative reverse transcription-PCR (qRT-PCR), Western blot, Flow cytometry analysis and Soft agar colony formation assays. (PDF 560 kb)