Additional file 1: of MICA immune complex formed with alpha 3 domain-specific antibody activates human NK cells in a Fc-dependent manner

Figure S1. FACS gating strategy for NK cytolytic activity assay. To analyze the NK cytolytic data acquired on FACSCalibur, target tumor cells were initially gated based on forward scatter (FSC) vs. side scatter (SSC). The CD56− target cell population was subsequently gated based on the dot plot of CD56-APC vs. 7-AAD to exclude contaminating NK cells; the 7-AAD+ target cell was finally gated within the CD56− gate to report target cell death as the percentage of 7-AAD+ cell population within the total CD56− cell population. (JPG 204 kb)