Additional file 1: of Long non-coding RNA TPTEP1 inhibits hepatocellular carcinoma progression by suppressing STAT3 phosphorylation

Figure S1. (A) Knockdown efficiencies of siRNAs against the ten indicated LncRNAs in QGY-7703 cells were detected by qRT-PCR. (B) Ratio of RPKM (reads per kilobase per million mapped reads) value from ribosomal profiling of each indicated gene in QGY-7703.(C and D) Relative RNA levels of TPTEP1 in QGY-7703 cells infected with the lentivirus expressing shRNA against TPTEP1 (shRNA-TPTEP1) (C), or in MHCC97H cells infected with the lentivirus expressing TPTEP1 (LV-TPTEP1) were detected by qRT-PCR (n=3; *represents P < 0.05). Figure S2. Cell proliferation (A) and invasive ability (B) were examined in shRNA-Control QGY-7703 cells and TPTEP1-knockdowned QGY-7703 cells (shRNA-TPTEP1-2, another TPTEP1 shRNA used to avoid off-target effects) (n=3; *represents P < 0.05). Figure S3. (A) STAT3 and p-STAT3 protein expressions in QGY-7703 cells infected with lenti-shRNA-TPTEP1 and then treated with cisplatinum (CS) were detected by Western blotting. (B) STAT3 and p-STAT3 protein expressions in the cytoplasmic and nuclear fractions in QGY-7703 cells infected with lenti-shRNA-TPTEP1 and then stimulated with IL-6 were detected by Western blotting (GAPDH as the cytoplasmic maker, and Histone H3 as the nuclear maker). Figure 4. (A) TPTEP1 expressions in IL-6 stimulated QGY-7703 cells were detected by qRTPCR. (B) STAT3 and p-STAT3 protein expressions in TPTEP1-knockdowned QGY-7703 cells transfected with shRNA-IL6 and then stimulated with IL-6 for 6 h were detected by Western blotting (n=3; *represents P < 0.05). Figure S5. BALB/c nude mice were divided into 3 groups randomly, and were injected with MHCC97H cells (NC), control MHCC97H cells (LV-Control) or TPTEP1-overexpressed cells (LV-TPTEP1). Five weeks later, the body weights of mice were monitored (n=6; *represents P < 0.05). Table S1. Clinicopathological Characteristics of the human samples used in this study. Table S2. Primers used in this study. (DOCX 321 kb)