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Additional file 1 of LiCoO2 particles used in Li-ion batteries induce primary mutagenicity in lung cells via their capacity to generate hydroxyl radicals

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posted on 2020-01-30, 04:57 authored by Violaine Sironval, Vittoria Scagliarini, Sivakumar Murugadoss, Maura Tomatis, Yousof Yakoub, Francesco Turci, Peter Hoet, Dominique Lison, Sybille van den Brule
Additional file 1: Figure S1. LCO and LTO particle endocytosis by RLE cells. Rat lung epithelial cells (RLE) were exposed to NaCl or 50 μg/ml of LCO or LTO and treated with cytochalasin B 4 h after particle exposure. Endocytosis was assessed 24 h after particle exposure. Images of binucleated cells after treatment. White arrows designate some particles (a). One hundred binucleated cells were scored to determine the proportion of cells containing particles (b) as well as the number of endocytosed particles per cell (c). Bars represent means ± SEM (N = 1, n = 2). Figure S2. Inflammatory dose-response to LCO and LTO particles. Wistar rats were treated with an oro-pharyngeal aspiration of NaCl (control, CTL), 0.1, 0.3, 1 or 5 mg LCO or LTO particles. Inflammation was assessed after 3 d. LDH activity (a) and proteins (b) were measured in the BALF, recruited inflammatory cells in the BAL (c). Bars represent means ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 relative to CTL mice (one-way ANOVA followed by a Dunnett’s multiple comparison, N = 1, n = 4).

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Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture (BE)

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