Additional file 1: of Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle

Figure S1. ΔKlotho mice display signs of sarcopenia. (A) Immunofluorescence of cross-sections of TA muscles from ΔKlotho and control mice stained for DAPI (DNA, blue) and Laminin (green) at p14, p21, and p56. Scale bar = 50 μm. (B) Minimal fiber feret measured on the whole cross-sections from ΔKlotho and control TA muscles at p14, p21, and p56. (p14, p56 n ≥ 3 mice per genotype, p21 n = 3 mice per genotype). (C) Quantification of the cross-section area of the mid-belly region of TA muscles determined from sections from ΔKlotho and control at p14, p21, and p56. (p14, p56 n ≥ 3 mice per genotype, p21 n = 3 mice per genotype). (D) Immunoblot analyses of atrophy associated ubiquitin ligases in TA muscles from ΔKlotho and control littermates at different ages. (E) Fold expression of different ubiquitin ligases as shown in (D), values for ΔKlotho are normalized to control animal of the same age. (F) Muscle weight of tibialis anterior (TA) muscles from adult ΔKlotho (n = 6) and control mice, n = 8 (G) Muscle weight of tibialis anterior (TA) muscles normalized to the length of the tibia bone from adult ΔKlotho (n = 6) and control mice (n = 8). All data are presented as means ± SEM. *p < 0.05, **p < 0.01, *** p < 0.001. (PDF 39024 kb)