Additional file 1 of Identifying epigenetic biomarkers of established prognostic factors and survival in a clinical cohort of individuals with oropharyngeal cancer

Additional file 1:Supplementary Figure 1. Heatmap showing correlation between top CpG sites (P<1x10-7) from each prognostic factor (alcohol consumption, HPV16 E6 seropositivity and smoking) and survival EWAS (Model 1: ~3-year survival adjusted for age sex and surrogate variables; Model 2: as Model 1, additionally adjusted for HPV16E6 seropositivity, smoking status and alcohol intake). Strength of association is shown by depth of colour; deeper red denotes a stronger phenotypic association with a hypermethylated CpG and deeper cyan denotes a stronger phenotypic association with a hypomethylated CpG. Supplementary Figure 2. - Surrogate variables correlated at P<0.05 (Pearson's) with laser surgery in HN5000. Supplementary Figure 3. - Surrogate variables correlated at P<0.05 (Pearson's) with surgery on an OPC primary tumour in HN5000. Supplementary Figure 4. - Surrogate variables correlated at P<0.05 (Pearson's) with neck resection surgery in HN5000. Supplementary Figure 5. - Surrogate variables correlated at P<0.05 (Pearson's) with teletherapy in HN5000. Supplementary Figure 6. - Surrogate variables correlated at P<0.05 (Pearson's) with chemotherapy in HN5000. Supplementary Figure 7. - Surrogate variables correlated at P<0.05 (Pearson's) with chemoradiotherapy in HN5000. Supplementary Figure 8. - Surrogate variables correlated at P<0.05 (Pearson's) with TNM stage in HN5000. Supplementary Figure 9. - Surrogate variables correlated at P<0.05 (Pearson's) with neutrophil-to-lymphocyte ratio in HN5000.