13046_2018_996_MOESM1_ESM.pdf (99.53 kB)
Additional file 1: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis
journal contribution
posted on 2019-01-06, 05:00 authored by Pei-Fang Hung, Tse-Ming Hong, Che-Chang Chang, Chung-Lieh Hung, Yuan-Ling Hsu, Yih-Leong Chang, Chen-Tu Wu, Gee-Chen Chang, Nei-Li Chan, Sung-Liang Yu, Pan-Chyr Yang, Szu-Hua PanFigure S1. Slug is primarily modified by SUMO-1. Slug is primarily SUMOylated by SUMO-1 in vivo. Lysates of HEK293T cells were cotransfected with plasmids encoding 3xFlag-tagged Slug and different GFP-tagged isoforms of SUMO. The lysates were collected and used for immunoprecipitation with an anti-Flag antibody. β-actin was used as the internal control. The asterisk and arrowhead indicate Slug modified and not modified by SUMO-1, respectively. (PDF 99 kb)
