Additional file 1 of Hypercholesterolemia negatively influences morphology and molecular markers of epithelial cells within the choroid plexus in rabbits

Additional file 1. AQP-1 immunohistochemical (IHC) staining of controls and dHC. A) Working concentration of rabbit polyclonal anti-AQP-1 antibody (0.5 μg/mL), B) normal rabbit IgG (0.5 μg/mL) control or C) PBS (primary antibody-omitted control) were used on CP sections from a rabbit fed a 0%-cholesterol diet. D) AQP-1 IHC staining on a different rabbit consuming a 0%-cholesterol diet than the rabbit in (A). Inset (e) is shown in a magnified field in (E). Apical side of CPECs are clearly AQP-1-positive. AQP-1 IHC staining from a dHC rabbit (F) with inset (g), which is magnified in (G). AQP-1 IHC staining in the CP (H) of another dHC rabbit. The AQP-1 stain of apical CPECs is weaker than that of 0%-cholesterol diet-fed rabbits. The LV CPs are shown.