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Additional file 1 of Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation
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posted on 2020-05-30, 03:45 authored by Monika Zilkova, Anna Nolle, Branislav Kovacech, Eva Kontsekova, Petronela Weisova, Peter Filipcik, Rostislav Skrabana, Michal Prcina, Tomas Hromadka, Ondrej Cehlar, Gabriela Paulikova Rolkova, Denisa Maderova, Michal Novak, Norbert Zilka, Jeroen J. M. HoozemansAdditional file 1. Supplementary Fig. S1 Characterization of heparin-induced tau oligomers. (A) FTIR spectroscopy of tau confirmed a structural change after in vitro fibrillization of human truncated tau151–391/4R. The prevalent disorder of monomeric tau (dashed line) changed to beta-rich structure (solid line). (B) Dynamic light scattering measurements showed high-molecular weight tau species in heparin-aggregated recombinant tau151–391/4R with an average radius spanning from 30 to 90 nm (solid line). Monomeric tau151–391/4R is included for comparison (dashed line).
