Additional file 1: of Human three-dimensional in vitro model of hepatic zonation to predict zonal hepatotoxicity

Figure S1. Viability of monolayer-cultured HepaRG cells after CHIR treatment (days 1, 3, 6, and 10). (A) Cell viability was evaluated using CCK-8 assays on days 1, 3, 6, and 10 after the CHIR treatment. (B) HepaRG cells was observed before and after 3 days of CHIR treatment under a phase-contrast microscope (scale bar, 100 μm). The microscopic observation showed that fully differentiated HepaRG cells were organized in small clusters and displayed a typical hepatocytes-like morphology. (TIF 2598 kb)