Additional file 1: of Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness

A: microscopic observation of C-FGSCs (colony-like FGSCs) and B-FGSCs (beaded-like FGSCs), the scale is 20 μm; B: DNA agarose electrophoresis of multi-stemness molecular markers; C: ALP staining of FGSCs, scale is 20 μm; D: double IF of Mvh and Oct4, scale is 20 μm; E: double IF of Mvh and EdU, the scale is 20 μm. (DOCX 320 kb)