Additional file 1: of Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 system

Figure S1. Agronomic performance of cv. YK17 and T2 generation tms5–1 and tms5–2 mutants grown during a normal growing season. Figure S2. Pollen fertility and grain set of cv. YK17 and the T3 generation mutants (YK17S1 and YK17S2) plants grown under various temperature regimes. Figure S3. Phenotype of cv. YK17, YK17S1, R106 and the F1 hybrid of YK17S1 x R106. Figure S4. Phenotype of cv. YK17, YK17S1, R207 and the F1 hybrid of YK17S1 x R207. Figure S5. Grain yield performance and yield components of cv. YK17, YK17S1, R106 and the F1 hybrid YK17S1 x R106. Figure S6. Grain yield performance and yield components of cv. YK17, YK17S1, R207 and the F1 hybrid YK17S1 x R207. Figure S7. PCR-based identification of T-DNA-free in F1 progenies of YK17S1 x R101 using primers directed at the Cas9 (A), gRNA scaffold (B) and hpt (C) sequence. Figure S8. PCR-based identification of T-DNA-free in F1 progenies of YK17S1 x R106 using primers directed at the Cas9 (A), gRNA scaffold (B) and hpt (C) sequence. Figure S9. PCR-based identification of T-DNA-free in F1 progenies of YK17S1 x R207 using primers directed at the Cas9 (A), gRNA scaffold (B) and hpt (C) sequence. Table S1. Detection of mutations in potential off-target sites in T-DNA-free T1 generation segregants. Nucleotides corresponding to the protospacer adjacent motif in each target site are shown in red. Mismatches are shown in blue and matches in black. Table S2. List of primers used in the present study. (PDF 1285 kb)