Additional file 1: of Gene length corrected trimmed mean of M-values (GeTMM) processing of RNA-seq data performs similarly in intersample analyses while improving intrasample comparisons

Smid, Marcel; Coebergh van den Braak, Robert; van de Werken, Harmen; van Riet, Job; van Galen, Anne; Weerd, Vanja de; van der Vlugt-Daane, Michelle; Bril, Sandra; Lalmahomed, Zarina; Kloosterman, Wigard; Wilting, Saskia; Foekens, John; IJzermans, Jan; Martens, John; Sieuwerts, Anieta

doi:10.6084/m9.figshare.6651653.v1

12859_2018_2246_MOESM1_ESM.pdf (189.97 kB)

Additional file 1: of Gene length corrected trimmed mean of M-values (GeTMM) processing of RNA-seq data performs similarly in intersample analyses while improving intrasample comparisons

journal contribution

posted on 2018-06-22, 05:00 authored by Marcel Smid, Robert Coebergh van den Braak, Harmen van de Werken, Job van Riet, Anne van Galen, Vanja de Weerd, Michelle van der Vlugt-Daane, Sandra Bril, Zarina Lalmahomed, Wigard Kloosterman, Saskia Wilting, John Foekens, Jan IJzermans, John Martens, Anieta Sieuwerts

Impact of gene length correction on correlation. Simulated expression data of 10 genes in 2 samples. Correlation based on read counts show different results after correcting for gene length. RPK indicates reads per kilobase. (PDF 189 kb)

Funding

KWF Kankerbestrijding

History

Usage metrics

Keywords

RNA sequencing Normalization methods GeTMM edgeR TPM DESeq2 Colorectal Cancer

Licence

CC BY + CC0

Exports

RefWorks

BibTeX

Ref. manager

Endnote

DataCite

NLM

DC

Additional file 1: of Gene length corrected trimmed mean of M-values (GeTMM) processing of RNA-seq data performs similarly in intersample analyses while improving intrasample comparisons

Funding

KWF Kankerbestrijding

History

Usage metrics

Categories

Keywords

Licence

Exports