Additional file 1: of FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma

Figure S1. Correlation between circ_002136, miR-138-5p and linear CDK11A and evaluation of the transfection efficiency. (A) The transfection efficiency of FUS. Data are presented as the means ± SD (n = 3, each group). **P < 0.01 vs. FUS (−) NC group. (B) The expression of circ_002136 with RNase R treatment. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. control group in ECs; ##P < 0.01 vs. RNase R group in ECs. (C) The expression of CDK11A with RNase R treatment. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. control group in ECs; ##P < 0.01 vs. control group in ECs. (D) The transfection efficiency of circ_002136 knockdown. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. circ_002136 (−) NC group. (E) The expression of CDK11A after circ_002136 knockdown. Data are presented as the means ± SD (n = 5, each group). (F) The transfection efficiency of CDK11A knockdown. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. sh-NC group. (G) The expression of circ_002136 after CDK11A knockdown. Data are presented as the means ± SD (n = 5, each group). (H) The transfection efficiency of miR-138-5p agomir or antagomir. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs. miR-138-5p (+) NC group, ##P < 0.01 vs. miR-138-5p (−) NC group. (I) The expression of CDK11A after miR-138-5p over-expression or silencing. Data are presented as the means ± SD (n = 5, each group). (J) The transfection efficiency of SOX13. Data are presented as the means ± SD (n = 3, each group). **P < 0.01 vs. SOX13 (+) NC group, ##P < 0.01 vs. SOX13 (−) NC group. (K) The transfection efficiency of SPON2. Data are presented as the means ± SD (n = 3, each group). **P < 0.01 vs. SPON2 (−) NC group. (TIF 471 kb)