Additional file 1: of Endogenous controls of gene expression in N-methyl-N-nitrosourea-induced T-cell lymphoma in p53-deficient mice

Stability analysis of reference genes in thymus by two additional primers. To rule out the possibility that the result was dependent on the specific primer used, two additional primers were designed for each reference gene. Sequence and amplification efficiencies, mean Ct values of the primers at each time point and result analyzed using geNorm and NormFinder were presented in the file. Figure S1. Range of quantification cycle values of the candidate reference genes. Mean of Ct values of primer 2 (A) and primer 3 (B) for the eight reference genes in thymus with or without MNU treatment at each time point. Figure S2. Expression stabilities of the eight candidate genes after MNU treatment in thymus. A, B. Mean expression stability values in thymus from least to most stable are presented on the y- and x-axes using geNorm (A) and NormFinder (B). Figure S3. Expression stabilities of the eight candidate genes during lymphoma development in thymus. A, B. Mean expression stability values in thymus from least to most stable expression are presented on the y- and x-axes using geNorm (A) and NormFinder (B). (DOCX 413 kb)