Additional file 1 of Empagliflozin prevents doxorubicin-induced myocardial dysfunction

Additional file 1.Extended methods. Extended version of study methodology, including all details that the limited word count available did not allow to keep in the main manuscript. Table S1. MicroRNAs targeting SGLT2. List of microRNAs potentially targeting SGLT2 within the highest context score percentiles. The upper row shows the microRNA targeting a conserved site of SGLT2, while the remaining microRNAs target poorly conserved sites. Figure S1. Study timeline. The figure depicts the study timeline with the breakdown of mice into three groups. Figure S2. Systemic blood pressure. At the sixth week, both systolic (panel A) and diastolic blood pressure (panel B) was significantly lower in the DOX group compared to DOX+EMPA. Figure S3. Histological examples of doxorubicin myocardial injuries. The figure shows the characteristic attenuation of fibrillar bands observed after treatment with doxorubicin, in some morphological variants (lower left and mid panels and upper mid panel). Masson’s trichrome staining highlights the degree of fibrosis in hearts from control group (upper right panel) and hearts from the DOX group (lower right panel). Figure S4. Expression of SGLT-1 in mice hearts. Results RT-PCR showing the expression levels of SGLT-1 in different mouse hearts. Figure S5. Expression of SGLT-2 in mice hearts. Immunohistochemistry shows the expression of SGLT-2 in mice hearts. SGLT-2 expression showed a 75% reduction in DOX mice (upper right panel), compared to baseline. This effect was significantly attenuated in EMPA+DOX mice (lower right panel).