Additional file 1: of Early herpes and TTV DNAemia in septic shock patients: a pilot study

Table S1. (A) Virus strain-reference panel and (B) viral PCR-reference panel used for determination of the LOD in the automated process. Figure S1. Selection of the so-called “viral cohort”, a subset of the MIP REA cohort. Figure S2. (A) Semi-automated procedure applied to detect DNA viremia in plasma. Internal controls are used all along the process to qualify each single step, namely extraction, amplification, detection. Figure S3. (A) Procedure applied to determine the LOD (B) Last performed dilution giving a positive count and selection (red) of the LOD (left), and amplification on blood extracts from healthy volunteers; a TTV LOD in plasma was derived from Kulifaj D. et al., 2018 ( https://doi.org/10.1016/j.jcv.2018.06.010 ). Table S2. Association between binary endpoints or markers and viremia presence. Clinical outcomes are mortality at D28 following ICU admission and HAI occurrence occurring during the hospitalization. Biomarkers, quantified by RT-PCR, consisted of CD74 ratio of D3/D1 (> 1.238 = increased incidence of HAI), CX3CR1 measured at D3 (> 0.253 = increased incidence of mortality at D28), IL10 measured at D3 (> 0.039 = increased incidence of HAI), and IL1b measured at D3 (increase at D2–D4 = increase incidence of HAI in pediatric patients). Thresholds were determined using the total MIPREA cohort, for CD74 and IL10 [16] and CX3CR1 as well [17, 19]. As no threshold was proposed for IL1β [19], it was used as a continuous variable. aIL1β not a binary endpoint. (PPT 1049 kb)