Additional file 1: of ELISA based on a recombinant Paragonimus heterotremus protein for serodiagnosis of human paragonimiasis in Thailand

Figure S1. Sizes of the inserted cDNA in randomly selected clones from the P. heterotremus cDNA library. PCR products resulting from amplification with the pJET forward primer and pJET reverse primer were separated on a 1.2% agarose gel. The sizes of inserted cDNA fragments from random clones (Lanes 1–14) were determined by comparison with the Kapa universal ladder (Lane M). (TIF 1102 kb)