Additional file 1: of E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway

Figure S1. (A) FoxM1 expression was higher in colon cancer tissues than in normal tissues, which is generated from UALCAN database. (B) According to UALCAN database, the Kaplan-Meier survival curve demonstrates high expression of FoxM1 in colon cancer correlated with poor survival. (C) shFoxM1 decreased β-catenin translocation to cell nuclei in Caco-2 cells, as immunofluorescence analysis shows. Nuclei were counterstained with DAPI. Magnification: 400×; Scale: 50 μm. (D) shFoxM1 decreased β-catenin in Caco-2 cell nuclei, as revealed by immunoblot analysis, with SP1 as loading control. TCF-1 and cyclin D1 expression was inhibited by shFoxM1. Right panel: Densitometric analysis of left normalized to GAPDH. *, P < 0.05. (JPG 261 kb)