Additional file 1: of Distribution of nickel and chromium containing particles from tattoo needle wear in humans and its possible impact on allergic reactions

Additional Methods. Figure S1. Nano-X-ray fluorescence (XRF) maps of four skin and three lymph node samples analyzed at ID16B. Figure S2. Nano-X-ray fluorescence (XRF) maps of selected inks analyzed at ID16B. Figure S3. Calculation of Ni and Cr contamination in pig skin and inks. Figure S4. T-cell infiltration in tattoo allergy sample. Table S1. Titanium XANES spectra of eight human skin and six lymph node samples as well as a skin allergy biopsy were fitted to pure anatase and rutile spectra of known standards. Table S2. MALDI-MS analysis of organic pigments in skin and lymph node samples. No pigments were found in the control samples. Table S3. Cr K-edge micro-XANES spectra of human skin and lymph node samples were fitted to spectra of known Cr standards. Table S4. Ni K-edge nano-XANES spectra of human skin and lymph node samples were fitted to known Ni standards. Table S5. ICP-MS analysis of elements in skin and lymph node samples. Increased values compared to skin or lymph node (LN) control samples are marked in bold. Table S6. Table-top X-ray fluorescence (XRF) analysis of microtome blades used for sample preparation and commercial tattoo needles. Tattoo needles analyzed derived from six different brands. Data are displayed as mean and standard deviation of n = 2 measurements. (DOCX 3000 kb)